當(dāng)前位置：杭州昊鑫生物科技股份有限公司>>云克隆>> SEA861Hu補(bǔ)體成分3(C3)檢測試劑盒
SEA861Hu
云克隆
代理商
杭州市
48T 1844元 10000盒可售
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| 產(chǎn)地 | 進(jìn)口 | 規(guī)格 | 48T |
|---|---|---|---|
| 級別 | 化工級 | 證書 | ISO系列證書 |
編號SEA861Hu
物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
實驗方法雙抗夾心
反應(yīng)時長3h
檢測范圍7.8-500ng/mL
靈敏度z小可檢測劑量小于等于3.7ng/mL.
樣本類型serum, plasma and other biological fluids
下載英文說明書 中文說明書
規(guī)格48T96T 96T*5 96T*10 96T*100
本試劑盒用于檢測補(bǔ)體成分3(C3)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
分別于定值血清及血漿樣本中加入一定量的補(bǔ)體成分3(C3)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-92 | 88 |
| EDTA plasma(n=5) | 87-102 | 92 |
| heparin plasma(n=5) | 93-101 | 97 |
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
在定值血清及血漿樣本內(nèi)加入適量的補(bǔ)體成分3(C3)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中補(bǔ)體成分3(C3)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 81-98% | 91-98% | 95-105% |
| EDTA plasma(n=5) | 91-105% | 89-98% | 99-105% | 88-102% |
| heparin plasma(n=5) | 79-93% | 89-96% | 89-96% | 78-92% |
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
將補(bǔ)體成分3(C3)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的補(bǔ)體成分3(C3)與連接于固相載體上的抗體結(jié)合，然后加入生物su化的補(bǔ)體成分3(C3)抗體，將未結(jié)合的生物su化抗體洗凈后，加入HRP標(biāo)記的親和素，再次洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成Z終的黃色。顏色的深淺和樣品中的補(bǔ)體成分3(C3)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
| 雜志 | 參考文獻(xiàn) |
| Artificial Organs | Experimental Research With Synthetic Copolymer-Coated Cardiopulmonary Bypass Circuits: Inflammatory and Thrombogenicity Analysis [Wiley: source] |
| Journal of Clinical Immunology | Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. [PubMed: 22941511] |
| Lipids in Health and Disease | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome [PubMed: PMC3480889] |
| Am J Reprod Immunol | Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo Study [PubMed: 26388133] |
| Veterinary Immunology and Immunopathology | Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin [Pubmed:27032499] |
| International Journal of Molecular Sciences | Identification of Serum Peptidome Signatures of Non-Small Cell Lung Cancer [Pubmed:27043541] |
| Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
| Scientific Reports | Generation of complement protein C3 deficient pigs by CRISPR/Cas9-mediated gene targeting. [pubmed:28694465] |
| Veterinary Microbiology | The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain. [pubmed:28888638] |
| Proteomics Clinical Applications | iTRAQ‐Based Proteomic Analysis Reveals Protein Profile in Plasma from Children with Autism [Pubmed:29274201] |
| Nanomaterials | An OMV-Based Nanovaccine Confers Safety and Protection against Pathogenic Escherichia coli via Both Humoral and Predominantly Th1 Immune Responses in … [Pubmed: 33233490] |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
| Scientific Reports | The bovine cumulus proteome is influenced by maturation condition and maturational competence of the oocyte [Pubmed: 32555221] |
| Int Immunopharmacol | Complement component 3 prevents imiquimod-induced psoriatic skin inflammation by inhibiting apoptosis in mice [Pubmed: 32535539] |
| Italian Journal of Animal Science | Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the … [] |
| Dev Comp Immunol | A deep-sea pathogenic Bacillus subtilis isolate employs different strategies to escape the killing of teleost and murine complements [33545212] |
| Animal | Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves [33610512] |
| British Journal of Pharmacology | The suppression of Brd4 inhibits peripheral plasma cell differentiation and exhibits therapeutic potential for systemic lupus erythematosus [] |
| Scientific Reports | Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes [34940989] |
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